Department of Pathology and Molecular Medicine, 2nd Faculty of Medicine, Charles University in Prague and Motol University Hospital
Ústav patologie a molekulární medicíny 2. LF UK a FN Motol
The Institute of Pathology and Molecular Medicine deals with complex laboratory diagnosis of tumorous and non-tumorous diseases.
We use methods of classical histology and cytology along with other related special laboratory examination methods. These methods include into-cells view and tissue at the ultrastructural level of analysis (electrone microscopy), expression of proteins demonstration (immunohistochemical methods, Western blot, flow cytometry), enzyme activity demonstration in tissue sections (enzyme histochemistry), analysis of changes at chromosomes and genes level, demonstration of certain viruses in histological section (methods of in situ hybridization) and an analysis of DNA and RNA extracted from tissues (methods of qualitative and quantitative polymerasis chain reaction, sequencing).
Examined biological material includes tissues (native, frozen, fixed), punctuates of bone marrow, peripheral blood or, as the case may be, other cellular fluids (transudates) and autologous grafts for bone marrow transplantations.
The most important tasks of the Institute
Our main task is ensuring a diagnosis and typization of diseases in patients who undergo an operation or patients, from whom samples of cells or tissues have been taken by means of a procedure other than surgical (endoscopic extractions, needle punctures, sponge biopsies of cells) in the University Hospital Motol. A part of examinations takes place within so-called preoperational biopsies which are carried out during operations and whose result influences surgeon’s decision making about a further course of the operation. A part of examinations carried out at the department serves for setting of a prognosis of certain diseases, for examining of response to treatment or for indication for a targeted specific therapy of patients. With its dissecting operation the department fulfills its educational and inspecting function within the University Hospital Motol and the 2nd Faculty of Medicine of Charles University in Prague.
The department also focuses on consulting activities with a wider range of effect within the Czech Republic, mainly for a diagnosis of child age tumors, for a diagnosis of diseases of lymphatic tissues (for lymphomas and lymphoproliferative diseases in general) and for diseases of the bone marrow within histological processing of tissues. Other areas of department specialization include: a diagnosis of kidneys diseases, a diagnosis of diseases of muscles and nerve system disorders – at the level of histopathology with the use of specialized laboratory procedures, a diagnosis of congenital developmental disorders, particularly of the cardiovascular system.
Diagnoses of diseases of various systems are dealt with by teams of trained professionals (doctors and biologists aided by laboratory workers) in specialized laboratories.
The Institute’s activities in instruction are summarized at the website of the 2nd Faculty of Medicine. The department is accredited for post-graduate training of doctors and histological laboratory workers.
Research and cooperation
The Institute of Pathology and Molecular Medicine focuses its research activities mainly on the areas of child age tumors, diseases of lymphatic tissues (lymphomas and lymphoproliferative states) diseases of the muscles and nerve system. In its research activities the Institute cooperates with clinics of the 2nd Faculty of Medicine and University Hospital Motol, with the Institute of Neurosciences of the 2nd Faculty of Medicine of Charles University and with the Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic. As a part of international cooperation, the Institute has had long-term relations with the following institutions: the Institute of Pathological Anatomy and the Consulting Center of Bioptic Diagnosis of Diseases of Blood Formation, Jessenius School of Medicine in Martin, Komenského University in Bratislava and University Hospital Martin and the Department of Neuropathology, Stanford University, Palo Alto, CA, USA.