Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital

Basic information

ÚLM laboratories provide bacteriological, virological, parasitological and mycological laboratory diagnostics using direct and indirect methods. Provides consulting and advisory services in the field of antibiotic therapy and differential diagnosis of infectious diseases for clinical workplaces of the Motol University Hospital. He cooperates with the Department of Hospital Hygiene and Epidemiology of the Motol University Hospital in the field of prevention of the spread of epidemiologically dangerous infections, including hospital ones. Through the antibiotic center, it influences rational antibiotic policy in practice.

ÚLM provides teaching of the subject of medical microbiology within the undergraduate education of the 2nd Faculty of Medicine, Charles University and within the postgraduate education in cooperation with the Department of Medical Microbiology, IPVZ and the 2nd Faculty of Medicine, Charles University. ÚLM has the following departments: departments of bacteriology, virology and molecular diagnostics, which include individual laboratory sections. They perform routine examinations of biological samples. It also performs highly specialized examinations to search for selected pathogens, mainly using molecular biological methods: the Cystic Fibrosis Laboratory, which works closely with the Cystic Fibrosis Center, Motol University Hospital in early detection and typing. B. cepacia a P. aeruginosa, the anaerobic infections department of the bacteriology department ensures molecular typing of strains Cl. difficult and the tissue culture laboratory of the virology department cooperate with the Department of Neurology in the determination of neutralizing antibodies against interferon beta.

The main mission of the ÚLM is to provide diagnostic laboratory activities, while the staff keep in mind that the benefit of the patient is a priority. Other activities such as pedagogical and scientific activities are organized in accordance with and in connection with routine laboratory diagnostics, without the risk of disrupting the medical and preventive focus of the workplace.

Management and staff

The head
prof. MUDr. Pavel Drevinek, Ph.D.
e-mail: pavel.drevinek@lfmotol.cuni.cz

Medical representative
MUDr. Otakar Nyc, Ph.D.
e-mail: otakar.nyc@fnmotol.cz

Senior Laboratory Technician
Vladimir Kryll
e-mail: vladimir.kryll@fnmotol.cz 

Clinic manager
M.Sc. Anna Pavlíková Kutějová
e-mail: anna.pavlikova@fnmotol.cz

Quality manager
Petra Koblová
e-mail: petra.koblova@fnmotol.cz

Secretariat
Romana Laušerová
e-mail: romana.lauserova@lfmotol.cuni.cz

Head of Department

Department of Serological Methods and Parasitology
MD Kamila Dundrová
e-mail: kamila.dundrova@fnmotol.cz 

Department of Molecular Methods
prof. MUDr. Pavel Drevinek, Ph.D.
e-mail: pavel.drevinek@lfmotol.cuni.cz

Department of Bacteriology and Mycology
MD Daniela Lžičařová
tel .: 224 435 388
e-mail: daniela.lzicarova@fnmotol.cz

ATB center
MUDr. Otakar Nyc, Ph.D.
e-mail: otakar.nyc@fnmotol.cz

Specialization

The department provides comprehensive bacteriological, virological, mycological and parasitological diagnostics using a wide range of diagnostic laboratory methods. Much attention is paid to the use of molecular genetic procedures in the diagnosis of infectious diseases, including both rapid methods for the earliest possible detection of infectious agents and panbacterial PCR with sequencing, applicable especially for infections caused by difficult or routinely non-cultivable microorganisms.

Specializes in early identification and typing of pathogens in patients with cystic fibrosis, diagnosis and epidemiology of infections Clostridium difficile, viral and fungal infections in patients with immunosuppressive therapy (including patients after various types of transplants) and for the detection of serious nosocomial pathogens with multiple resistance to antibiotic preparations. Great emphasis is placed on the appropriate interpretation of microbiological findings, including consultation, which, among other things, includes the recommendation of antimicrobial treatment, especially in critically ill and immunodeficient patients. Part of the workplace is an antibiotic center striving to promote the principles of rational antibiotic treatment into practice.

The institute is an undergraduate and postgraduate teaching institution and is accredited for the field of medical microbiology. Research and publication activities are focused on the evaluation of new methods for the diagnosis of infectious diseases, updating or formulation of recommended procedures for the diagnosis and treatment of infectious diseases, the epidemiology of multi-resistant pathogens and the clarification of mechanisms of antimicrobial resistance.

The structure of the institute

Department of Virology

The ÚLM Department of Virology deals with a wide range of diagnostics of viral infections based on both serological indirect detection and direct viral detection using antigen detection or molecular biology.

The main focus is on viral infections in immunosuppressed patients, especially in patients after allogeneic hematopoietic stem cell transplantation and patients after lung and kidney transplants. In these groups of patients, opportunistic viral infections play a significant role in increasing morbidity and mortality, which is why we have been involved in various projects for a long time addressing the importance of detecting individual viruses for the patient's clinical condition. That is why we try to expand the range of provided examinations, in addition to the long-term detection of DNA viral infections (herpesviruses, polyomaviruses, adenoviruses, parvovirus B19), also by detecting RNA viruses, especially respiratory viruses (Influenza, RSV, hMPV,…). The laboratory of molecular genetics of the Pediatric Clinic of the 2nd Medical Faculty of Charles University and the Motol University Hospital (www.lmg.cz) also deals with this issue.

Recently, we have also been focusing more significantly on the resistance of human cytomegalovirus (CMV) and herpes simplex virus (HSV) in particular to therapeutically administered antiviral drugs.

Part of our work is also the detection and quantification of hepatitis C virus.

Thanks to the knowledge of classical virological techniques, the detection of antibodies against interferon β, administered therapeutically in patients with multiple sclerosis, is also carried out in our laboratory in cooperation with the Department of Neurology.

Close cooperation with most clinics, especially with the Department of Pediatric Hematology and Oncology, then allows us to more effectively assess viral detection in terms of its impact on the patient's condition and prognosis.

Antibiotic center

The Institute of Medical Microbiology includes the Antibiotic Center, whose activities have a long tradition in the Motol hospital. In addition to consulting in the field of antibiotic treatment, categorization and approval of antimicrobials, it proposes and participates in recommendations concerning some special areas of antiinfectives such as surgical prophylaxis, febrile neutropenia, etc. antibiotic resistance and to maintain the effectiveness of the widest possible range of antibiotics within the Motol University Hospital.

Mycology laboratory

 

Since 1.12.2009 December XNUMX, the Institute of Medical Microbiology has also included an independent mycology laboratory, where we mainly diagnose invasive fungal infections.

We offer conventional microscopic and culture detection of invasive fungal infections, and we verify susceptibility to antifungal drugs in detected pathogens. We also examine respiratory samples for the presence of Pneumocystis jiroveci by PCR. The treatment of fungal infections is consulted with the ATB Center of the ÚMM.

In cooperation with the Department of Pediatric Hematooncology, Motol University Hospital, we analyze the incidence of invasive fungal infections in significantly immunocompromised patients and participate in the CELL project (Czech leukemia study group for life) and the ECMM (European Confederation for Medical Mycology) invasive zygomycosis study.

Microbiological examination of airway samples from patients with cystic fibrosis

Diagnosis is focused on the detection of common or new infections in patients with cystic fibrosis (CF).

The physiological properties of bacterial isolates from CF patients can differ significantly from the characteristics typical of individual species, which significantly limits their detection and determination of their susceptibility to antibiotics. The most well-known phenomena are eg dissociation of bacterial culture into several morphotypes with different antibiograms, occurrence of mutants (auxotrophs) requiring the addition of growth factor in the growth medium, occurrence of hypermutant strains with original resistance mechanisms or mucosal phenotype in case of biofilm formation in vivo. Direct detection and clonal characterization of chronic pathogens (Pseudomonas aeruginosa, Burkholderia cepacia complex) by amplifying specific DNA from the sample and analysis by typing methods will allow early initiation of specific therapy and implementation of practical anti-epidemic measures in case of epidemic strains during outpatient visits or hospitalization of CF patients. In addition to the classic infectious agents in patients with CF (S. aureus, haemophilus spp, P. aeruginosa a B. cepacia complex) diagnostics also deals with new pathogens such as genus representatives Pandora.

Laboratory staff are members of international activities dealing with CF issues (eg European Cooperation in Science and Technology). The activities of the laboratory are directly connected with the activities of the Center for Cystic Fibrosis at the Pediatric and Pneumology Clinic at the Motol University Hospital, where they care for patients with CF.

The auxotrophic S. aureus strain requires growth factor in the culture mediumThe auxotrophic strain of S. aureus requires growth ...
Growth of the mucosal strain P. aeruginosa on blood agarGrowth of the mucosal strain P. aeruginosa on ...
Growth of B. cenocepacia on diagnostic agarGrowth of B. cenocepacia on diagnostic agar
Final analysis of bacterial strains of S. aureusFinal analysis of bacterial

Documents to download

Laboratory manuals

For the professional public

Research

  • Clostridium difficile - The laboratory focuses on the detection of toxins Clostridium difficile in the examined sample, culturing the strain and determining the susceptibility to the antibiotics of choice. The presence of toxins is verified in parallel C. difficile state-of-the-art genetic methods, including ribotyping of grown cultures.
  • Cystic fibrosis - microbiological diagnostics in patients with cystic fibrosis and characterization of pathogens using phenotypic and genotypic methods in close cooperation with the Center for Cystic Fibrosis at the Pediatric Clinic of the University Hospital in Motol.

Publications

 Employees of the department regularly present data at domestic and international congresses and publish them in magazines.

 List of foreign publications that have been prepared by us since 2007:

  • Alterations of CHEK2 forkhead-associated domain increase the risk of Hodgkin lymphoma.
    Havranek O., Spacek M., Hubacek P., Mocikova H., Markova J., Trneny M., Kleibl Z.
    Neoplasma. 2011;58(5):392-5.
  • Havranek O., Spacek M., Hubacek P., Mocikova H., Benesova K., Soucek P., Trneny M., Kleibl Z.
    No association between the TP53 codon 72 polymorphism and risk or prognosis of Hodgkin and non-Hodgkin lymphoma.
    Leuk Res. 2011 2011 Aug; 35 (8): 1117-9.
  • Spacek M., Hubacek P., Markova J., Zajac M., Vernerova Z., Kamaradova K., Stuchly J., Kozak T.
    Plasma EBV-DNA monitoring in Epstein-Barr virus-positive Hodgkin lymphoma patients.
    APMIS. 2011 Jan;119(1):10-6.
  • King L., Stuchlý J., Hubáček P., Keslová P., Sedláček P., Starý J., Hrušák O., Kalina T.
    Signature profiles of CMV-specific T-cells in patients with CMV reactivation after hematopoietic SCT.
    Bone Marrow Transplant. 2010 Aug; 46 (8): 1089-98
  • Spacek M., Hubacek P., Markova J., Zajac M., Vernerova Z., Kozak T.
    Can we use EBV-DNA monitoring to predict disease relapse in EBV-positive hodgkin lymphoma patients?
    Acta Haematol. 2010;124(1):23-6.
  • Bojar M., Zajac M., Meluzínová E., Houžvičková E., Libertinová J., Lišková P., Mat'oška V., Nyč O., Mináriková M.
    Treatment with azathioprine and cyclic methylprednisolone has little or no effect on bioactivity in anti-interferon beta antibody-positive patients with multiple sclerosis.
    Mult Scler. 2010 Dec; 16 (12): 1529-30; author reply 1531-2. Epub 2010 Sep 9.
  • Hubáček P., Virgili A., Ward KN, Pohlreich D., Keslová P., Goldová B., Marková M., Zajac M., Cinek O., Nacheva EP, Sedláček P., Cetkovský P.
  • HHV-6 DNA throughout the tissues of two stem cell transplant patients with chromosomally integrated HHV-6 and fatal CMV pneumonitis.
    Br J Haematol. 2009 May; 145 (3): 394-8.
  • Hubáček P., Mužíková K., Hrdličková A., Cinek O., Hynčicová K., Hrstková H., Sedláček P., Starý J.
    Prevalence of HHV-6 integrated chromosomally among children treated for acute lymphoblastic or myeloid leukemia in the Czech Republic.
    Journal of Medical Virology. J Med Virol. 2009 Feb; 81 (2): 258-63.
  • Hubacek P., Keslova P., Formankova R., Pochop P., Cinek O., Zajac M., Lochmanova J., Stary J., Sedlacek P.
    Cytomegalovirus encephalitis / retinitis in allogeneic haematopoietic stem cell transplant recipient treated successfully with combination of cidofovir and foscarnet.
    Pediatrician Transplant. 2008 Nov 1.
  • Sedlacek P., Formankova R., Mejstrikova E., Keslova P., Hubacek P., Dobrovolna M., Vrana M., Kupkova L., Pittrova H., Stary J.
    Allogeneic stem cell transplantation in children with leukemia using human leukocyte antigen-mismatched unrelated donors.
    Pediatrician Transplant. 2008 Feb; 12 (1): 24-31.
  • Hubacek P., Sedlacek P., Keslova P., Formankova R., Stary J., Kulich M., Cinek O.
    Incidence of HHV7 in donors and recipients of allogeneic hematopoietic stem cell transplantation.
    Pediatrician Blood Cancer. 2008 Apr; 50 (4): 935
  • Hubáček P., Maalouf J., Zajíčková M., Kouba M., Cinek O., Hynčicová K., Fales I., Cetkovský P.
    Failure of multiple antivirals to affect high HHV-6 DNAaemia resulting from viral chromosomal integration in case of severe aplastic anemia.
    Haematologica. 2007 Oct;92(10):e98-e100.
  • Hubacek P., Hyncicova K., Muzikova K., Cinek O., Zajac M., Sedlacek P.
    Disappearance of pre-existing high HHV-6 DNA load in blood after allogeneic SCT.
    Bone Marrow Transplant. 2007 Oct; 40 (8): 805-6. Epub 2007 Aug 20.
  • Janda A., Sedlacek P., Mejstrikova E., Zdrahalova K., Hrusak O., Kalina T., Sieglova Z., Zizkova H., Formankova R., Keslova P., Hubacek P., Sediva A., Bartunkova J ., Dlask K., Stary J.
    Unrelated partially matched lymphocyte infusions in a patient with complete DiGeorge / CHARGE syndrome.
    Pediatrician Transplant. 2007 Jun; 11 (4): 441-7.

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Contact

In Úval 84
150 06 Prague 5
Tel.: 224 435 390
e-mail: microbiologie@fnmotol.cz
location: BUILDING 20

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