Department of Transplants and Tissue Banks

Basic information

Department of Transplants and Tissue Banks

FN Motol creates optimal conditions for the implementation of organ and tissue procurement and transplantation, especially with its comprehensive personnel and instrumentation.

The EATB.CZ Civic Association has been involved in the cardiovascular tissue collection, processing and transplantation program since 2004. Its chairman is MUDr. Jaroslav SPATENKA, CSc.

Tax ID CZ26660148

Website: www.eatb.cz

Account number: 0138861399 / 0800

Primary

MD Jan BURKERT, Ph.D.

Secretariat

(224) 433 002
ottb@fnmotol.cz

Management and staff

Knowledge

MD Jan BURKERT, Ph.D.

primary

tel .: +420 224 433 000

fax: +420 257 221 056

Email: jan.burkert@fnmotol.cz

MUDr. Jaroslav SPATENKA, CSc.

deputy mayor

tel .: +420 224 433 000

fax: +420 257 221 056

Email: jaroslav.spatenka@fnmotol.cz

Anna HABRMANOVA, CETC, CTBS

head nurse

tel .: +420 224 433 005

fax: +420 257 221 056

Email: anna.habrmanova@fnmotol.cz

Secretariat

Bc. Ing. Andrea DOBOŠOVÁ 

technical worker, quality manager

tel .: +420 224 433 002

 fax: +420 257 221 056

Email: andrea.dobosova@fnmotol.cz

Coordination workplace

Transplant center

Tissue equipment

Procurement program, processing, investigation, release, storage and distribution

Cardiovascular tissue bank

MUDr. Jan BURKERT, Ph.D. 

responsible person 

responsible for the preparation, processing, storage and allocation of allografts of heart valves and blood vessels

tel .: +420 224 433 000 fax: +420 257 221 056

Email: jan.burkert@fnmotol.cz

MUDr. Jaroslav SPATENKA, CSc. 

responsible person 

responsible for the preparation, processing, storage and allocation of allografts of heart valves and blood vessels

tel .: +420 224 433 000 fax: +420 257 221 056

Email: jaroslav.spatenka@fnmotol.cz

Musculoskeletal tissue bank 

MD Rastislav BALLAY 

responsible person , responsible for the collection, processing and release of bone grafts from living donors

I. orthopedic clinic of the 1st Medical Faculty of Charles University and University Hospital Motol

 tel .: +420 224 433 933, +420 224 433 955 

 Email: rastislav.ballay@fnmotol.cz

MUDr. Jiří STEINDLER

responsible person, responsible for the collection, processing and release of autologous bone grafts

Department of Neurosurgery for Children and Adults, 2nd Medical Faculty, Charles University and University Hospital Motol

tel .: + 420 224 432 551

Email: jiri.steindler@fnmotol.cz

Dagmar HRABANKOVÁ

tissue bank specialist

tel .: +420 224 433 003 fax: +420 257 221 056

Email: dagmar.hrabankova@fnmotol.cz

Teresa CHRZ

tissue bank specialist 

tel .: +420 224 433 003 fax: +420 257 221 056

Email: tereza.chrz@fnmotol.cz

Bank of other tissues

Doc. Mgr. Katerina JIRSOVA, Ph.D.

responsible person

responsible for the procurement, processing and release of other tissues

tel .: +420 224 433 007 fax: +420 257 221 056

Email: katerina.jirsova@fnmotol.cz

Mgr. Denisa Nemetova, Ph.D.

responsible person

responsible for the procurement, processing and release of other tissues

tel .: +420 224 433 003 fax: +420 257 221 056

Email: denisa.nemetova@fnmotol.cz

Programs

Organ procurement and transplantation program

Organ and tissue procurement

Tissue collection and transplantation program - Special tissue bank - STB85 / code MzČR /

The code STB 85 (Specialized Tissue Bank) was assigned to our TZ by the Ministry of Health. We deal with the collection and processing of cardiovascular tissue, the collection and processing of bone tissue from living and deceased donors and the collection of placentas for the subsequent preparation of amniotic membrane grafts.

TZ works on the basis of an activity permit issued by the State Institute for Drug Control. This institute also performs regular supervision of the activities of the tissue facility.

TZ STB85 in its activities is governed by applicable legislation, ie Act. No. 285/2002 as amended (the so-called "Transplantation Act") and especially Act. No. 296/2008 Coll. vol. Human Cells and Tissues Act ”as amended. Its activities are therefore in accordance with Directives 2004/23 / EC, 2006/17 / EC and 2006/86 / EC of the European Parliament and of the Council. The Annual Report on the Activities of TZ is published annually (see Documents to download).

Cardiovascular tissue bank

The program of collection, processing and transplantation of cardiovascular tissue (heart valves and blood vessels) is implemented mainly by TC OTTB employees, although legislatively it is a tissue program. Cardiovascular procurement is most often performed as part of multiorgan procurement in an organ transplant program throughout the country in cases where for some reason the donor's heart cannot be transplanted as an organ and blood vessels were not collected at the same time as organ grafts (liver, pancreas, kidneys).  

The offer of a potential heart or blood vessel donor is accepted by the OTTB FN Motol transplant coordinator from the KST service, from which, if accepted, they will also learn the results of serological screening of the deceased donor. The transport of the collected hearts and blood vessels and their further processing is organized by the transplant coordinator, acute surgical processing is provided by cardiovascular surgeons from the Department of Cardiovascular Surgery, Motol University Hospital.

Microbiological examination of tissues during processing is provided by the Institute of Medical Microbiology. Cryopreservation is carried out in the leased premises of UHKT Prague. The allocation of grafts and their release for use is the responsibility of the responsible person of the bank. The distribution of valve and vascular grafts for transplantation is provided by the coordinators according to the requirements of cardiovascular surgeons.

Grafts are delivered to the Children's Cardiac Center and the Cardiovascular Surgery Clinic at the Motol University Hospital. Cardiac surgeons at individual cardiac surgeries throughout the Czech Republic regularly order and transplant valve grafts. The Cardiovascular Tissue Bank holds a renewed export and import permit from the Ministry of Health of the Czech Republic every year (for all WHO countries).

Musculoskeletal tissue bank

A bone donor bank was established in 2004 at the Motol University Hospital Transplant Center, which was authorized by the Ministry of Health of the Czech Republic (see history).

Currently, this bank is part of TZ OTTB and has a SÚKL permit for musculoskeletal tissue (bone, ligament, cartilage) from living donors for autologous and allogeneic use in the scope of activities: procurement, processing, investigation, release, storage, distribution, laboratory investigation donors to assess fitness and donor selection. For musculoskeletal tissue from deceased donors, SÚKL is licensed for the following activities: procurement, processing, investigation, release, storage and distribution. We are talking here about those deceased donors who have been included in the national organ and tissue donor program.

Microbiological examination of tissues during processing is provided by the Institute of Medical Microbiology. Testing of donors as part of the assessment of their medical fitness is performed by the Institute of Medical Chemistry and Clinical Biochemistry of the 2nd Medical Faculty of Charles University and the Motol University Hospital or the Institute of Laboratory Methods IKEM Prague.

Grafts for autologous use: these are autologous bone grafts - parts of the calf, taken during decompression craniectomy. After the cerebral edema subsides, patients are implanted with cranioplasty.

Grafts for allogeneic use are delivered at FN Motol to the 1st Orthopedic Clinic of the 2st Faculty of Medicine, Charles University and Motol University Hospital, the Department of Pediatric and Adult Orthopedics and Traumatology of the 1nd Faculty of Medicine, Charles University and Motol University Hospital and to the Department of Spondylosurgery, XNUMXst Faculty of Medicine, Charles University and Motol University Hospital. Based on individual needs, bone grafts are prepared and delivered to dental surgeries.

Bank other tissues

This workplace currently holds a SÚKL permit for the preparation of amniotic membrane grafts for allogeneic use in the scope of activities performed: procurement, processing, investigation, release, storage and distribution.

The tissue preparation portfolio consists of frozen and dried amniotic membrane grafts and frozen and dried amniochorion membrane grafts.

The grafts are delivered to the Ophthalmology Clinic of the General Hospital in Prague and the 1st Medical Faculty of Charles University and to the Ophthalmological Clinic of the Royal Vinohrady University Hospital. The ingestion of these grafts in patients with long-term non-healing wounds is in preparation.

About the workplace

Department of Transplantation and Tissue Bank, Motol University Hospital (OTTB) is an independent department of the Motol University Hospital, which coordinates the activities of participating clinics, departments and complements of the Motol University Hospital and the 1st and 2nd Medical Faculties of Charles University in Prague so that an effective organ and tissue procurement program can be implemented at the Motol University Hospital and individual organ and tissue transplantation programs.

The sophisticated structure of the workplace serves this goal - see organisational scheme.

The management of the workplace, the secretariat and the coordinating workplace ensure the work of both fundamentally different organizational units:

• Transplant Center (TC)

ensures organ procurement and transplantation, cooperates with the Coordination Center for Transplantation (KST), with the Ministry of Health and is legislatively governed by Act No. 285/2002 Coll. as amended (the so-called Transplantation Act). The KST is audited within the scope of regulations and recommendations of the European Union.

• Tissue equipment (TZ)

ensures tissue collection and transplantation, cooperates with KST, with the Ministry of Health and is legislatively governed by Act No. 295/2008 Coll. as amended (the so-called Cell and Tissues Act). This law delegates control activities to the State Institute for Drug Control (SÚKL), which regularly audits all TZ programs.

Organizational structure of the workplace

Documents to download

Annual reports (statistics) for download:

History

Kidney transplant program in children

Since 1980, a hemodialysis program has been available at the Motol University Hospital for pediatric patients, dr. For many years, Špatenka provided monopoly access routes to the circulation for children in general and for hemodialysis in particular.

Since 1981, at the request of IKEM (Prof. V. Kočandrle, MD, DrSc.), The children's kidney transplant program (which began in 1977) has been transferred from IKEM to the Motol University Hospital. In 1981, the first pediatric kidney transplant was performed here. The program was provided by the First Children's Clinic, Children's Cardiac Center, Department of Pediatric Surgery and Department of Anaesthesiology and Resuscitation. Since 1982, Dr. Spatenka invited to all meetings of the so-called Transplant Commission. It was a body that consisted of the heads of Czechoslovak transplant centers and managed the transplant program in the country. The transplant commission was convened by prof. Kočandrle, head of the IKEM Transplant Center and head of the international transplant organization "Intertransplant", which united the renal transplant programs of the then Czechoslovakia, GDR and MLR.

At the suggestion of doc. Jandy and Dr. Špatenky was recognized by the Ministry of Health of the Czechoslovak Socialist Republic in the 80s at the Children's Dialysis and Transplant Center at the First Children's Clinic.

In 1991, the Ministry of Health of the Czechoslovak Republic appointed a new Transplant Commission, to which all heads of Transplant Centers were nominated. In the same year, the director of FN Motol dr. Kryl also set up a "Children's Transplant Center" in our hospital, which was headed by Dr. Spatenka. At that time, the center provided only organ harvesting within the Motol University Hospital and a program of kidney transplants in children for the whole of Czechoslovakia. This program continues successfully to the present. Even after the division of Czechoslovakia, small children from Slovakia are transplanted here.

Tissue bank

In 1992, Kryobanka of the heart valve allografts was established at the Children's Transplant Center by the director of the Motol University Hospital. The bank was partially equipped with the Z 112 grant "Production and preservation of heart valves and blood vessels for cardiovascular surgery", which was solved in the years 1991-1994. . The cryo-warehouse with nitrogen management was established on the wing of the 1 D children's monoblock. In 2003, a new Motol CryoWarehouse was built on the premises of HB3 from the hospital's Research Plan. One of the three cubicles has a transplant center. In the new cryostore, valve and vascular grafts are stored in liquid nitrogen, under conditions that meet EU accreditation requirements.

In 2004, a group of employees of the XNUMXst Orthopedic Clinic of the Motol University Hospital under the leadership of MUDr. Jan HACHA in order to build the Bank of Musculoskeletal Tissue. The grafts are also stored in a new cryostore.

In accordance with the Transplantation Act, the Ministry of Health of the Czech Republic issued a decision in November 2004 approving the establishment of the Specialized Tissue Bank, which assigned the registration number STB85.

The bank is currently authorized to collect, process, store and distribute cardiovascular tissues from deceased donors and musculoskeletal tissues from living donors.

Lung transplant program

The Motol University Hospital transplant center participated in the preparation of a lung transplant program for III. surgical clinic before moving this workplace to the Motol University Hospital. In 1997, the General FN Transplant Center (led by Prof. Patko) was formally merged with the Motol FN Children's Transplant Center, which has since lost only a pediatric focus and is called the Motol FN Transplant Center. Cooperation with AHK Vienna was deepened, a number of employees III. surgical clinic completed training with prof. Klepetka and in December 1997 the first lung transplant was performed in the Czech Republic. This program is being further developed successfully.

Organ and tissue procurement program

Organ and tissue procurement has been carried out at the Motol University Hospital since 1977. Due to the fact that the Children's Transplant Center did not have (unlike other transplant centers) a procurement region, there were only a few procedures per year.

After the abolition of the Czech transplant in 1998, MUDr. Vojtěch Martínek, CSc., Former director of the Czech transplant and prof. MUDr. Karel Matoušovic, DrSc., His deputy to the Motol University Hospital. Both are European-level nephrologists with extensive experience working abroad!

Both work at the Internal Medicine Clinic and the Transplant Center uses their expertise and international contacts in transplant medicine and especially in the field of organ and tissue procurement. MUDr. Martinek, CSc. has very successfully taken over the management of the organ and tissue procurement program. During this time, the number of beds at the Department of Anaesthesiology and Resuscitation and the number of intensive care beds where patients are ventilated has increased. We managed to increase the number of donations to the level of other transplant centers in the country (per million inhabitants). After the departure of Dr. Martinka, CSc. from FN Motol was the head of this program from January 1, 2005 to August 1, 8 prof. MUDr. Karel Matousovic, DrSc.

Gallery

Routes for remote organ harvesting

Tissue bank

Certificates